Bacillus sp. PS35 Lipase-Immobilization on Styrene-Divinyl Benzene Resin and Application in Fatty Acid Methyl Ester Synthesis

bacillus sp. ps35 lipase-immobilization on styrene-divinyl benzene resin and application in fatty acid methyl ester synthesis

background: lipase is an enzyme with immense application potential. ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. objective: the objectives of the study were to immobilize the lipase from bacillus sp. ps35 by cross-linking and adsorptio...

synthesis of 4,4’-isopropylidene diphenol with cation-exchange resin catalyst based on polystyrene-divinyl benzene

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Synthesis and Characteristics of Mesoporous Sol-gels for Lipase Immobilization

Enzyme cost is the major problem for industrial scale application. Immobilization is a promising approach to moderate the enzyme cost factor and increase its stability and activity. In this study, sol-gel method was used to prepare the immobilization platform and entrapped lipase as one of the most used enzyme in dairy processing, cosmetics and pharmaceutical industries. Lipase from Candida rug...

Immobilization and stability studies of a lipase from thermophilic Bacillus sp: The effect of process parameters on immobilization of enzyme

A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60oC and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30% v/v each). The activity (assayed by determining the release of pNP from pNP laurate) was stimulated up to 60% of these solvents in enzyme reaction mixture. The c...

FATTY ACID METHYL ESTER PROFILING OF Enterococcus and E

Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase

BACKGROUND Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, as a solution aid, would reduce the productivity of the enzyme. Esterification, a solvent-free and no-glycerol-release system for biodiesel production, has been developed, and two-step catalysi...